Infection by burrowing nematode causes toppling disease of banana, yellows disease of pepper and spreading decline of citrus. Quarantine requirements for the importation of fresh. Rearing of radopholus slwlis on banana fruit callus stephen m. Radopholus similis, the burrowing nematode, is the most commonly found pest of arecanut sundararaju and koshy, 1988. Cn102382819a dna extract of radopholus similis in morbid. Although control of this pest has mainly relied on the use of clean planting material, reinfestation of plants in the field remains a critical concern. Acta horticulturae home login logout status help ishs home ishs contact. Helicotylenchus multicinctus cobb thome is also widely distributed. It is an especially important pest of bananas and citrus, and it can be found on coconut, avocado, coffee, sugarcane, other grasses, and ornamentals. The histological changes in banana roots caused by radopholus similis and helicotylenchus multicinctus published on 01 jan 1966 by brill.
Serine carboxypeptidases scps are peptidases that hydrolyse peptides and proteins and play critical roles in the development. Exploring the transcriptome of the burrowing nematode. The european commission requested that the efsa panel on plant health perform a pest categorisation for radopholus similis and radopholus citrophilus. Kropff in the presence of the thesis committee appointed by the academic board. Pdf in vitro tolerance and resistance to burrowing. Estimation of damage caused by the burrowing nematode. We retrieved 7380 est sequences consisting different tissuescondition libraries from dbest of national centre for biotechnology information ncbi. These two races were later split into two separate species based on morphological, biochemical, and cytological differences. Genomic diversity between radopholus similis populations from around the world detected by rapdpcr analysis published on 01 jan 1996 by brill. Meeting minutes of the working group on ditylenchus.
Rearing of radopholus similis on banana fruit callus. Radopholus similis distribution in anthurium plant tissue was determined in a greenhouse experiment. Genetic algorithm for ruleset prediction modeling system garp and. This page was last edited on 17 january 2020, at 00. Radopholus similis radosihost plants eppo global database. In a survey of plant parasitic nematodes of native vegetation in new zealand four radopholus species were found. The dna extract contains the following components with final concentration. Three 3 inoculum levels of radopholus similes 50, 250 and 500 nematodes were employed. Susceptibility of banana intercrops for rhizobacteria.
Studies on the life history and habits of the burrowing nematode, radopholus similis, the cause of blackhead disease of banana. A sikora 3 fungal endophytes from bananas for the biocontrol of radopholus similis b. Sikora2 introduction the burrowing nematode radopholus similisis one of the major pathogens affecting banana production worldwide and is considered the primary pathogen of banana root. Ochieno thesis submitted in partial fulfilment of the requirements for the degree of doctor at wageningen university by the authority of the rector magnificus prof.
Ishs international symposium on the culture of subtropical and tropical fruits and crops preplant treatment of radopholus similis in infested banana land. Radopholus similis is a species of nematode known commonly as the burrowing nematode. Eradication of the burrowing nematode, radopholus similis, from bananas. The burrowing nematode of bananas radopholus similis. Fiche informative radopholus citrophilus et radopholus. Yang menyerang tanaman kopi please copy and paste this embed script to where you want to embed. Quarantine requirements for the importation of propagation.
Com worksheetanswer key a simile is a fun way to compare something. While every effort has been made to provide the most reliable and uptodate information available, ultimate legal requirements with respect to species are contained in. In this publication, we present the genome assembly of r. Pdf twenty populations of radopholus similis from three countries and different hosts 19 populations from. The burrowing nematode radopholus similis is one of the main quarantine pests in china, and the risk of invasion posed by this nematode is becoming more. Text is available under the creative commons attributionsharealike license. Radopholus similis and meet the requirements outlined in state exterior. The nematode was for a period described as a separate species, radopholus citrophilus, but has now been shown to be a different biological isolate, the citrus race. It is a parasite of plants, and it is a pest of many agricultural crops. The burrowing nematode radopholus similis is one of the main quarantine pests in china, and the risk of invasion posed by this nematode is becoming more and more serious with regard to the international trade being intensified day by day. Data sheet on radopholus citrophilus and radopholus similis. A person with a bad sunburn can be described this way. Fungal endophytes from bananas for the biocontrol of.
Categories have been assigned by the eppo secretariat on the basis of available data at the time of entry. The biological control efficacy of single or multiple applications of the mutualistic endophyte fusarium oxysporum strain 162, the egg pathogen paecilomyces lilacinus strain 251 and the antagonistic bacteria bacillus firmus toward radopholus similis was investigated in pot trials with banana under glasshouse conditions. Unraveling the transcriptome of this migratory plantparasitic nematode can provide insight in the parasitism process and lead to more efficient control measures. Burrowing nematode, radopholus similis cobb 1893 thorne. Morphological differences among radopholus simile cobb, 1893 thorne, 1949 populations. Radopholus similis also known as the burrowing nematode is a devastating pest of banana musa spp. Susceptibility of banana intercrops for rhizobacteria, arbuscular mycorrhizal fungi and the burrowing nematode radopholus similis. They correspond to an evaluation of the biological importance of the host plant for the pest concerned, together with the economic importance of this plant for the eppo region. It is a migratory endoparasite of roots, causing lesions that form cankers. Infested consignments are expected to enter the risk assessment area on ornamentals under. The potential geographic distribution of radopholus.
Radopholus similis an overview sciencedirect topics. Pdf identifikasi ras fisiologi nematoda radopholus. Download adobe acrobat reader free software to read pdf files. Quarantine requirements for the importation of propagation materials which are hosts of radopholus similis from the netherlands quarantine requirements for the importation of propagation materials which are hosts of radopholus similis from the netherlands pdf file size. For the first high throughput molecular characterization of this devastating nematode, 5,853 expressed sequence tags from a mixed stage population were. Radopholus similis is a migratory endoparasite, spending its adult vermiform life in the root and corm tissues where it completes its life cycle in 2025 days, but is also capable of emerging in adverse conditions. However, it is not a legal authority for statutory or regulatory purposes. The histological changes in banana roots caused by. References this secernentea roundworm related article is a stub.
It is urgent to analyse the potential geographic distribution of r. Radopholus similis is one of the key pests of banana in the east african highlands and in the world. Pest risk assessment of radopholus similis for the eu. In vitro tolerance and resistance to burrowing nematode, radopholus similis, in anthurium species.
Proceedings of the helminthological society of washington, 29. The objective of this study is to explore the single sequence repeats ssrs and single nucleotide polymorphims snps in expressed sequence tags ests of radopholus similis. The quantitative assessment focused on entry, establishment, spread and impact on tropical and subtropical ornamental host plants, the main pathways for entry of r. Radopholus similis using fusarium oxysporum v5w2 in tissue culture banana dennis m.
Biological control of radopholus similis in banana by. Ishspromusa symposium on recent advances in banana crop protection for sustainable production and improved livelihoods molecular diagnostics in the mycosphaerella leaf spot disease complex of banana and for radopholus similis. They are highly destructive and damage a wide range of plants by destroying the cortex as they feed in. Six candidate reference genes were chosen from the transcriptome database of radopholus similis using the bioinformatics method, including four conventional reference genes actin, eukaryotic translation initiation factor 5a eif5a, tubulin alpha atubulin, ubiquitin ubi and two new candidate reference genes ribosomal protein s21 rps21 and serinethreonine protein phosphatase. Achlysiella williamsi is a plant pathogenic nematode in the genus saccharum which parasitizes sugarcane saccharum officinarum. Radopholus similis that does not infect citrus and radopholus citrophilus that can infect citrus huettel. Itis taxonomy is based on the latest scientific consensus available, and is provided as a general reference source for interested parties. Information about radopholus similis diagnosis, including distribution and treatment advice. Radopholus similis using fusarium oxysporum v5w2 in tissue. Description of the banana nematode radopholus similis ecology, natural distribution, development cycle and pathotypes, the damage. Mining ssr and snpindel sites in expressed sequence tag. Pra scientific opinion on the pest categorisation of. Vessey radopholus similis cobb thorne is an important pathogen of bananas in most bananaproducing coun tries obannon, 1977.
Pdf morphological and karyotypic differences within and among. This page was last edited on 7 january 2019, at 14. Radopholus similis is an important parasitic nematode of plants. Radopholus similis the banana knowledge platform of the. Intercultivating these crops in arecanut gardens increases the nematode infestation sundararaju and koshy, 1988. Population dynamics and economic threshold of the nematodes radopholus similis and pratylenchus goodeyi on banana in australia. The migratory endoparasite, radopholus similis, is a pest to over 250 plant species, including economically important crops such as banana and citrus haegeman et al. The panel on plant health performed a pest risk assessment on radopholus similis, the burrowing nematode for the eu.
Ditylenchus dipsaci, and rootknot nematodes meloidogyne spp. The potential geographic distribution of radopholus similis in china. The species exhibits a pronounced sexual dimorphism. Top of page radopholus citrophilus, previously regarded, at least in some quarters, as a separate species. Radopholus similis is an important migratory endoparasitic nematode, severely harms banana, citrus and many other commercial crops. The invention discloses a dna extract of radopholus similis in morbid plant tissues, an extraction method and application thereof, and relates to a molecular biology detection technology of plant diseases.
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